مقالات  دكتر ماريا ظهيري  

  Encapsulation of curcumin loaded chitosan nanoparticle within poly (ε-caprolactone) and gelatin fiber mat for wound healing and layered dermal reconstitution. Encapsulation of curcumin loaded chitosan nanoparticle within poly (ε-caprolactone) and gelatin fiber mat for wound healing and layered dermal reconstitution. . 2020 Jun 15;153:1241-50. ISI 5.16 Zahiri M, Khanmohammadi M, Goodarzi A, Ababzadeh S, Farahani MS, Mohandesnezhad S, Bahrami N, Nabipour I, Ai J
Abstract Hybrid electrospun fiber containing bioactive molecules, which offer the ability to deliver the cells into the wound bed, will help to achieve a high therapeutic effect. In this study, an electrospun polycaperlactone (PCL) and gelatin (Gela) scaffold containing curcumin loaded chitosan nanoparticle (NCs/Cur) was used to evaluate in vivo wound healing ability of the fabricated scaffolds. The electrospun hybrid scaffold seeded with human endometrial stem cells (EnSCs) showed desirable biocompatibility with the host immune system and wound healing ability in a full-thickness excisional animal model. The constructs were characterized for structural, mechanical and biochemical properties. Fourier transform infrared spectroscopy (FTIR) confirmed all typical absorption characteristics of PCL and Gela polymers as well as NCs and Cur. The results showed the perfect contact angle, wettability and degradability of hybrid fiber scaffolds with the good mechanical and structural characteristics including shape uniformity, pore size and porosity. The cell attachment and proliferation on the PCL/Gela/NCs/Cur was higher than PCL and PCL/Gela scaffolds. In term of the capability of hybrid scaffold and EnSCs in histological analysis, this novel tissue-engineered construct could be suggested as a skin substitute to repair injured skin and regenerative medicine application.


  Dimethyloxalylglycine preconditioning enhances protective effects of bone marrow-derived mesenchymal stem cells in Aβ-induced Alzheimer disease Physiology & behavior. 2019 Feb 1;199:265-72. ISI 2.690 Esmaeilzade B, Artimani T, Amiri I, Najafi R, Shahidi S, Sabec M, Farzadinia P, Zare M, Zahiri M, Asl SS
Abstract Mesenchymal stem cell (MSC) transplantation therapy has been proposed as a promising approach for the treatment of neurodegenerative disease. Chemical and pharmacological preconditioning before transplantation could optimize the therapeutic properties of transplanted MSCs. In this study, we hypothesized that preconditioning treatment with a prolyl hydroxylase inhibitor, dimethyloxalylglycine (DMOG), will increase MSC efficacy and paracrine effects in an amyloid-β (Aβ)-injected Alzheimer rat model. MSCs were incubated in different concentrations of DMOG for 24 h. Cell viability, migration, and antioxidant capacity was assessed in DMOG-treated and non-treated MSCs before transplantation into Aβ-injected rats. In vitro analysis revealed that DMOG treatment increased cell viability, migration, and expression of CXCR4, CCR2, Nrf2, and HIF-1α in the MSCs. Our in vivo results show that DMOG preconditioning enhances a MSC-mediated rescue of learning and memory function in Aβ-injected rats. Furthermore, we found an increased level of BDNF and total antioxidant capacity in the hippocampus of Aβ-injected rats following transplantation of preconditioned relative to untreated MSCs. Our results suggest that preconditioning MSCs with DMOG before transplantation may enhance the efficacy of stem cell based therapy in neurodegenerative disease.


  Stem cells in review.. ISMJ. 2014 Oct 10;17(4):733-47 Zahiri M, Shafikhodaii S, Keshavarz H.

  High porous electrospun poly (ε‐caprolactone)/gelatin/MgO scaffolds preseeded with endometrial stem cells promote tissue regeneration in full‐thickness skin wounds: An in vivo study. Journal of Biomedical Materials Research Part B: . Applied Biomaterials. 2020 May9 ISI 2.674 َbabzadeh S, Farzin A, Goodarzi A, Karimi R, Sagharjoghi Farahani M, EslamiA.
Abstract In the current study, electrospun poly(ε‐caprolactone)‐gelatin (PCL‐Gel) fibrous scaffolds containing magnesium oxide (MgO) particles and preseeded with human endometrial stem cells (hEnSCs) were developed to use as wound care material in skin tissue engineering applications. Electrospun fibers were fabricated using PCL‐Gel (1:1 [wt/wt]) with different concentrations of MgO particles (1, 2, and 4 wt%). The fibrous scaffolds were evaluated regarding their microstructure, mechanical properties, surface wettability, and in vitro and in vivo performances. The full‐thickness excisional wound model was used to evaluate the in vivo wound healing ability of the fabricated scaffolds. Our findings confirmed that the wounds covered with PCL‐Gel fibrous scaffolds containing 2 wt% MgO and preseeded with hEnSCs have nearly 79% wound closure ability while sterile gauze showed 11% of wound size reduction. Our results can be employed for biomaterials aimed at the healing of full‐thickness skin wounds.


  Scintigraphic evaluation of remote pre-conditioning protection against unilateral renal ischemia/reperfusion injury in rats: a longitudinal study International urology and nephrology. 2019 Nov 1;51(11):2083-9 ISI 1.620 Sedaghat Z, Fatemikia H, Tanha K, Zahiri M, Assadi M.
Abstract Purpose To determine the role of remote perconditioning (RPeC) on renal function and histology in an animal model of unilateral renal ischemia and reperfusion (IR) injury. Methods Rats were subjected to 60 min unilateral renal ischemia. RPeC protocol was the application of four cycles of 5 min IR of left femoral artery during renal ischemia. Assessments of histological changes and renal function were made 24 h, 1 week, or 3 weeks later. 99mTc-DMSA scan was performed using a small-animals SPECT system. Results 24-h reperfusion decreased the 99mTc-DMSA uptake in the left kidney compared to the intact kidney of control animals. RPeC group has higher uptake compared to the IR group. After 1 week and 3 weeks, uptakes were gradually increased in both groups and no differences were observed. Severe morphological changes in the ischemic kidneys of both groups were observed after 24 h which attenuated after 1 week and 3 weeks. Moreover, no differences in creatinine and BUN levels between IR-treated and intact animals were observed. Conclusion These data suggest that RPeC exerts a partially transient improvement in the renal function in the first day after reperfusion. However, long-term follow-up study showed no beneficial effects of RPeC. Moreover, noninvasive 99mTc-DMSA scan revealed a suitable tool in the follow-up evaluation of recovery process in the unilateral renal IR injury models.


  Antioxidant effects of alpha-tocopherol (vitamine E) on testis regeneration in busulfan-treated mice ISMJ. . 2016 Sep 10;19(4):511-25 Rohnavaz F, Mirzapour T, Bayrami A, Zahiri M.

  Use of Stem Cells in the Treatment of Myocardial Infarction. Journal of Fasa University of Medical Sciences. 2020 Jun 10;10(2):2206-25 Zahiri M, Parviz S.

  Nerve Growth Factor and the Trans-differentiation of Human Dental Pulp Stem Cells Into Cholinergic-like Neurons . Basic and Clinical Neuroscience. - 2019 Nov 10;10(6):7 ISI 1.67 Darabi S, Tiraihi T, Nazm Bojnordi M, Ghasemi Hamidabadi H, Rezaei N, Zahiri M, Alizadeh R.
Introduction: Cell therapy has been widely considered as a therapeutic approach for neurodegenerative diseases and nervous system damage. Cholinergic neurons as one of the most important neurons that play a significant role in controlling emotions, mobility, and autonomic systems. In this study, human dental pulp stem cells (hDPSCs) were differentiated into the cholinergic neurons by β-mercaptoethanol in the preinduction phase and also by the nerve growth factor (NGF) in the induction phase. Methods: The hDPSCs were evaluated for CD73, CD31, CD34, and Oct-4. Concentration-time relationships for NGF were assessed by evaluating the viability rate of cells and the immune response to nestin, neurofilament 160, microtubule-associated protein-2, and choline acetyltransferase. Results: The hDPSCs had a negative response to CD34 and CD31. The optimal dose for the NGF was 50 ng/mL seven days after the induction when the highest percentage of expressing markers for the cholinergic neurons (ChAT) was detected. Conclusion: The results of this study provided a method for producing cholinergic neurons by hDPSCs, which can be used in cytotherapy for degenerative diseases of the nervous system and also spinal cord injury.



  Successful Transplantation of Frozen-thawed Calf Spermatogonial Stem Cells to the Mouse Testis Journal of Isfahan Medical School. 2013 Jan 15;30(213). Rahimi-Feyli P, Tajik P, Koruji M, Shafiei S, Zahiri M

  Role of cerebrospinal fluid in differentiation of human dental pulp stem cells into neuron-like cells. Anatomy & cell biology. . 2020 Sep 30;53(3):292 ISI 1.5 Goudarzi G, Hamidabadi HG, Bojnordi MN, Hedayatpour A, Niapour A, Zahiri M, Absalan F, Darabi S
Abstract Human dental pulp stem cells (hDPSCs) could be differentiated into neuron like-cells under particular microenvironments. It has been reported that a wide range of factors, presented in cerebrospinal fluid (CSF), playing part in neuronal differentiation during embryonic stages, we herein introduce a novel culture media complex to differentiate hDPSCs into neuron-like cells. The hDPSCs were initially isolated and characterized. The CSF was prepared from the Cisterna magna of 19-day-old Wistar rat embryos, embryonic cerebrospinal fluid (E-CSF). The hDPSCs were treated by 5% E-CSF for 2 days, then neurospheres were cultured in DMEM/F12 supplemented with 10-6 μm retinoic acid (RA), glial-derived neurotrophic factor and brain-derived neurotrophic factor for 6 days. The cells which were cultured in basic culture medium were considered as control group. Morphology of differentiated cells as well as process elongation were examined by an inverted microscope. In addition, the neural differentiation markers (Nestin and MAP2) were studied employing immunocytochemistry. Neuronal-like processes appeared 8 days after treatment. Neural progenitor marker (Nestin) and a mature neural marker (MAP2) were expressed in treated group. Moreover Nissl bodies were found in the cytoplasm of treated group. Taking these together, we have designed a simple protocol for generating neuron-like cells using CSF from the hDPSCs, applicable for cell therapy in several neurodegenerative disorders including Alzheimer’s disease. Keywords: Human dental pulp stem cells, Cerebrospinal fluid, Cell transdifferentiation, Alzheimer

doi: 10.5115/acb.19.241

  Trans-Differentiation of Human Dental Pulp Stem Cells Into Cholinergic-Like Neurons Via Nerve Growth Factor Basic and Clinical Neuroscience.. 2019 Nov;10(6):609 Darabi S, Tiraihi T, Bojnordi MN, Hamidabadi HG, Rezaei N, Zahiri M, Alizadeh R.

  The effect of oral Aphanizomenon flos-aquae extract on excisional wound healing. Tehran University Medical Journal TUMS Publications. 2019 Dec 10;77(10):646-50 Zahiri M, Pourkhalili K, Darvishi S, Heydari H, Akbari Z.

  Expression profile of germ stem cell-specific genes in human spermatogonial stem cells after co culture with sertoli cells ISMJ. . 2014 May 10;17(2):150-60 Zahiri M, Movahedin M, Mowla SJ, Noruzinia M, Noroozi MR, Amirjanati N.

  Antimicrobial peptides-loaded smart chitosan hydrogel: Release behavior and antibacterial potential against antibiotic resistant clinical isolates International Journal of Biological Macromolecules. 2020 Dec 1;164:855-62 ISI 5.162 Rezaei N, Hamidabadi HG, Khosravimelal S, Zahiri M, Ahovan ZA, Bojnordi MN, Eftekhari BS, Hashemi A, Ganji F, Darabi S, Gholipourmalekabadi M.
Abstract In this study, we synthesized thermo-responsive chitosan (TCTS) hydrogels, and loaded with different concentrations of antimicrobial peptide (AMP) (0, 4, 8 and 16 μg·ml−1) to fabricate an antibacterial wound dressing against resistant clinical isolates. Physico-chemical properties, release behavior, cytobiocompatibility and antibacterial activity of the AMP-TCTS hydrogels against standard strain and resistant Acinetobacter baumannii were fully determined in vitro. The TCTS-40% β-glycerolphosphate hydrogels showed a gelation time of 15 min at 37 °C. 80% weight loss at day 35 with no changes in pH value was observed. AMP-TCTS hydrogels showed a burst release of AMP (around 40%) at day 1, and a controlled release up to day 7. A dramatic water uptake was observed at first 4 h, and then continued for 10 h in a steady manner. All the AMP-TCTS hydrogels showed excellent cytobiocompatibility for human fibroblasts. The TCTS showed no antibacterial activity against both standard strain and clinical isolates. All the AMP-TCTS hydrogels had strong antibacterial activity against standard strains, but only 16 μg·ml−1 showed antibacterial behavior against resistant A. baumannii. Our results strongly suggest the 16 μg·ml−1 AMP-TCTS hydrogel as an excellent antibacterial wound dressing against resistant A. baumannii, and now promises to proceed with pre-clinical investigations.


  Metformin-Loaded PCL/PVA Fibrous Scaffold Preseeded with Human Endometrial Stem Cells for Effective Guided Bone Regeneration Membranes ACS Biomaterials Science & Engineering- Publication Date: December 21, 2020 ISI 4.152 Lida Ebrahimi, Ali Farzin, Younes Ghasemi, Aliakbar Alizadeh, Arash Goodarzi, Arefeh Basiri, Maria Zahiri, Ahmad Monabati, Jafar Ai
Regardless of approaches for treating damaged bone tissues, a beneficial Abstract Abstract Image Many studies have been devoted to investigating the potential of guided bone regeneration (GBR) membranes for bone defect reconstruction.therapeutic strategy has remained a challenge. In this study, a novel GBR membrane with polycaprolactone (PCL) and poly(vinyl alcohol) (PVA) containing different concentrations of metformin (Met) for improving osteogenic properties was developed. The membranes were evaluated for their hydrophilicity, degradation rate, swelling ratio, drug release, mechanical properties, and biological responses. The results showed a significant increase in hydrophilicity, swelling ratio, and degradation rate and no significant changes in mechanical properties of PCL/PVA membranes with Met concentration enhancement. A decrease in cell viability cultured on the surface of the PCL/PVA membrane was seen when the amount of Met was changed from 10 to 15 wt %. The results of the in vitro quantitative real-time polymerase chain reaction (qRT-PCR) also confirmed the higher secretion of osteogenic-related genes in a PCL/PVA/Cell/10 wt % Met scaffold than in the PCL/PVA/Cell sample. Therefore, further in vivo studies were conducted using the electrospun PCL/PVA membrane containing human endometrial stem cells (hEnSCs) and 10% Met. Histopathological and histomorphometric results confirmed that PCL/PVA/hEnSCs/10 wt % Met has excellent potential to differentiate hEnSCs into osteogenic lineages and bone regeneration in calvarial defects of rats. The results of this study confirm the high potential of the PCL/PVA/10 wt % Met fibrous membrane preseeded with hEnSCs in GBR applications.


  The Epigenetic Assessment and Proliferation of Human Spermatogenic Cells Derive from Obstructive Azoospermic Patients in Different Culture Systems Urology Journal. 5 January 2019 , Page 6092 1.378 Zahiri M, Movahedin M, Mowla SJ, Noruzini M, Koruji M, Nowroozi MR, Bashiri Z
Abstract Purpose: Generating functional gametes for patients with male infertility is of great interest. We investigated different cultural systems for proliferation of SSCs derived from obstructive azoospermic patients. Materials and Methods: Testicular cells were obtained from men with obstructive azoospermia. after enzymatic digestion process, cells assigned to various groups: culture of SSCs in the dish without cover (control group), co-culture of SSCs with infertile Sertoli cells (I), co-culture of SSCs with fertile Sertoli cells (II), culture of SSCs on nanofiber (covered with laminin) (III), culture of testicular cell suspension (IV). Then cells were cultured and evaluated colony formation, gene-specific methylation by MSP, quantitative genes expression of pluripotency (Nanog, C-Myc, Oct-4) and specific germ cell (Integrin α6, Integrin β1, PLZF) genes in five different culture systems. Results: Our findings indicate a significant increase in the number and diameter of colonies in IV group in compare to control group and other groups. Expression of germ specific genes in IV group were significantly increased (P≤0.05) and levels of expression of pluripotency genes were significantly decreased in this group (P≤0.05) compared with other groups. Gene-specific pattern of methylation of examined genes showed no changes in culture systems during the culture era. Conclusion: A microenvironment capable of controlling the proliferation of cell colonies can be restored by testicular cell suspension.

DOI: 10.22037/uj.v16i7.6092

  . The methanol extract of red algae, Dichotomaria obtusata, from Persian Gulf promotes in vitro osteogenic differentiation of bone marrow mesenchymal stem cells; a biological and phytochemical study. . Journal of Pharmacy and Pharmacology 2021 Jan 13 ISI 2.571 Nekooei M, Shafiee SM, Zahiri M, Maryamabadi A, Nabipour I
Abstract Objectives Osteoporosis is a major public health problem that is appeared with increasing age. This study evaluated the effect of the algae Dichotomaria obtusata methanol extract on osteogenic differentiation of the cultured bone marrow mesenchymal stem cells (BMMSCs) in vitro and analyzed the algae methanol extract to find out the potent beneficial components. Methods Dichotomaria obtusata were collected from the coastal area of Bushehr City in the Persian Gulf, Iran. The expression of osteogenesis-related genes was examined using real-time PCR. The formation of calcium deposits in differentiated MSCs was examined by Alizarin R staining. Analyses of algae extract ingredients were performed by gas chromatography–mass spectrometry (GC–MS). Key findings Methanol extract of the algae caused the up-regulation of osteogenic genes that were significant for Osteopontin and Osteocalcin (P < 0.05) and also led to an increase in calcium deposits and matrix mineralization in BMMSCs. The GC–MS analyses of the algae extracts resulted in the identification of steroids and essential fatty acids. Conclusion The results of the study indicated that the methanol extract of D. obtusata may possess significant potentials for the prevention of osteoporosis in vitro.


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