Enhancing rat full-thickness skin wounds with a mixed aloe/chitosan gel
Formosan Journal of Surgery
Zeinab Janahmadi, Meisam Rezaei Motlagh, Sasan Zaeri
Background: Finding optimal treatment for skin wounds, especially using natural substances, has been of main focus in skin care and cosmetics. Aloe vera has been historically used in wound care, and chitosan, which is obtained from crustaceans, has a variety of biomedical applications. Accordingly, it seems that a mixture of aloe/chitosan may provide added benefits in wound healing.
Aims: The aim of the present study was to evaluate the effects of a mixture of aloe/chitosan gel in comparison to each component alone, on the healing of excisional full-thickness wounds in rats.
Materials and Methods: Round full-thickness wounds were made on the back of animal necks. Five experimental groups received either no treatment (Group 1) or daily topical treatments with 1% carboxymethyl cellulose (CMC) (Group 2), aloe (Group 3), chitosan (Group 4), or mixed aloe/chitosan gels (Group 5) for 14 days. Wound areas on days 3, 7, and 14 and histopathologic parameters on day 14 were measured for analysis.
Results: Means of wound areas showed a significant decreasing pattern in all groups through days 3–14 (P < 0.001). The topical treatment of wounds with aloe/chitosan significantly reduced wound areas compared to nontreated (P = 0.019) or 1% CMC-treated (P = 0.021) wounds. Histopathologic analysis revealed more reepithelialization, fibroblasts, collagen fibers and less polymorphonuclear leukocytes, new vessels, and granulation tissue in wounds treated with aloe/chitosan in comparison to controls (P < 0.05).
Conclusions: Aloe/chitosan-treated wounds showed convincing healing profiles affirming the benefits of using natural bioactive substances in wound healing, especially in combination therapy.
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The Effect of Adding Alginate Natural Polymer on the Structure of Polyvinyl Alcohol Biocompatible Nanofibers in Electrospinning Process
Iran South Medical Journal
Maryam Najafiasl , Shahriyar Osfouri, Reza Azin, Sasan Zaeri
4 از 4
Background: Nowadays, in order to preserve the environment and sustainable development, the use of natural and renewable resources is a priority for industries. High performance and specific structure of nano-biocompatible materials has attracted researchers. In this research, alginate polymer, which is
generally obtained from marine sources such as algae, was added to polyvinyl alcohol nanofibers in order to improve their biocompatibility and investigate the effect of solution parameters on their morphology.
Materials and Methods: Polyvinyl alcohol nanofibers were produced in different concentrations by electrospinning to investigate the effect of concentration, adding alginate polymer and Triton x-100 surfactant. The quality of nanofibers and rheology behavior of polymer solutions were evaluated using scanning electron microscopy, rheometry and tensiometer.
Results: The results showed that increasing the concentration of polymer solution increased the diameter of nanofibers, as well as the viscosity of the polymer solution, such that a polyvinyl alcohol solution with 10wt.% concentration produced more uniform nanofibers. At first, adding alginate to the polyvinyl alcohol solution deteriorated electrospinning, however, production of nanofibers was improved by increasing polyvinyl alcohol solution to 10wt.%. Also, the results showed that adding Triton x-100 surfactant to the polymer solution affected the dominant mechanism in electrospinning by reducing surface tension and viscosity of the solution at polyvinyl alcohol solution 6wt.% and 10wt.%, respectively.
Conclusion: Under optimized conditions of solution parameters, suitable nanofibers will be produced, which will be applicable in many industries, such as drug delivery.
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