مقالات  دكتر بنفشه اسماعيل زاده  


  Early stereological changes in liver of Sprague-dawly rats after streptozotocin injection.Indian Journal of Gastroenterology Indian Journal of Gastroenterology 2005 vol 24 May-june p,104-107 ISI 5 1- Noorafshan A, Esmailzadeh B, Bahmanpour S, Poost-pasand A 2 4
Background: Diabetes mellitus is associated with biochemical, physiological and pathologic alterations in the liver. We measured changes in structure of rat liver after streptozotocin injection, using stereology. Methods: Livers of 36 streptozotocin-injected rats were removed after 4, 8 and 12 weeks. Liver volume and weight were measured, and volume-weighted mean volume of hepatocytes and their nuclei were estimated in periportal (Z1), interstitial (Z2) and perivenous (Z3) zones of liver acini. Volume of liver sinusoids was also estimated. Results: Mean volume and weight of the liver were reduced by 15% and 12%, respectively at 4 and 8 weeks after injection. Mean hepatocyte volumes were reduced by approximately 30%, 31% and 24% in Z1, Z2 and Z3 at 4 weeks, 19% and 24% in Z2 and Z3 at 8 weeks, and 14% in Z1 at 12 weeks. Mean volume of hepatocyte nuclei was reduced by approximately 18% and 20% in Z2 and Z3 at 4 weeks, 23% in all three zones at 8 weeks, and 18%, 15% and 13% in Z1, Z2 and Z3, respectively, at 12 weeks. The absolute volume of the sinusoids decreased by 16.5% only at 4 weeks. Conclusion: Streptozotocin injection leads to early reduction in volume of hepatocytes, their nuclei and sinusoids in rat liver. [Indian J Gastroenterol 2005;24:104-107]

دریافت فایل پیوست

Indian Journal of Gastroenterology 2005 vol 24 May-june p,104-107

  Neuropathological Changes in Brain Cortex and Hippocampus in a Rat Model of Alzheimer’s Disease . IBJ 15(1 & 2), 51-58. (2011) 15(1 & 2), 51-58. (2011) ISI 5 Nobakht M, Hosseini, S. M., Mortazavi, P., Sohrabi, I., Esmaeilzade, B 5 7
Background: Alzheimer’s disease (AD) is a neurodegenerative disorder with progressive loss of cognitive abilities and memory loss. The aim of this study was to compare neuropathological changes in hippocampus and brain cortex in a rat model of AD. Methods: Adult male Albino Wistar rats (weighing 250-300 g) were used for behavioral and histopathological studies. The rats were randomly assigned to three groups: control, sham and β-amyloid (Aβ) injection. For behavioral analysis, Y-maze and shuttle box were used, respectively at 14 and 16 days post-lesion. For histological studies, Nissl, modified Bielschowsky and modified Congo red staining were performed. The lesion was induced by injection of 4 μL of Aβ (1-40) into the hippocampal fissure. Results: In the present study, Aβ (1-40) injection into hippocampus could decrease the behavioral indexes and the number of CA1 neurons in hippocampus. Aβ injection CA1 caused Aβ deposition in the hippocampus and less than in cortex. We observed the loss of neurons in the hippocampus and cerebral cortex and certain subcortical regions. Y-maze test and single-trial passive avoidance test showed reduced memory retention in AD group. Conclusion: We found a significant decreased acquisition of passive avoidance and alternation behavior responses in AD group compared to control and sham group (P<0.0001). Compacted amyloid cores were present in the cerebral cortex, hippocampus and white matter, whereas, scattered amyloid cores were seen in cortex and hippocampus of AD group. Also, reduced neuronal density was indicated in AD group. Iran. Biomed. J. 15 (1 & 2): 51-58, 2011 Keywords: Alzheimer's disease (AD), Hippocampus, β-amyloid (Aβ), Neuropathological changes

دریافت فایل پیوست

IBJ 15(1 & 2), 51-58. (2011)

  Delivery of Epidermal Neural Crest Stem Cells (EPI-NCSC) to hippocamp in Alzheimer's Disease Rat Model Iranian Biomedical Journal 16 (1): 1-9 (January 2012) ISI 5 Banafshe Esmaeilzade1,2, Maliheh Nobakht*3,4 , Mohammad Taghi Joghataei1, Nahid Rahbar Roshandel5, Homa Rasouli1, Ali Samadi Kuchaksaraei6, Seyed Mohammad Hosseini7, Nowruz Najafzade8, Sara Asalgoo1, Leila Beygom Hejazian1 and Fatima Moghani Ghoroghi1 1 11
Background: Alzheimer’s disease (AD) is characterized by progressive neuronal loss in hippocamp. Epidermal neural crest stem cells (EPI-NCSC) can differentiate into neurons, astrocytes and oligodendrocytes. The purpose of this study was to evaluate the effects of transplanting EPI-NCSC into AD rat model. Methods: Two weeks after induction of AD by injection of Amyloid-β 1-40 into CA1 area of rat hippocamp, Y-maze and single-trial passive avoidance tests were used to show deficit of learning and memory abilities. EPI-NCSC were obtained from the vibrissa hair follicle of rat, cultured and labeled with bromodeoxyuridine. When Alzheimer was proved by behavioral tests, EPI-NCSC was transplanted into CA3 area of hippocamp in AD rat model. The staining of EPINCSC markers (nestin and SOX10) was done in vitro. Double-labeling immunofluorescence was performed to study survival and differentiation of the grafted cells. Results: We showed that transplanted EPI-NCSC survive and produce many neurons and a few glial cells, presenting glial fibrillary acidic protein. Total number of granule cells in hippocamp was estimated to be more in the AD rat model with transplanted cells as compared to AD control group. We observed that rats with hippocampal damage made more errors than control rats on the Y-maze, when reward locations were reversed. Conclusion: Transplanted cells were migrated to all areas of hippocamp and the total number of granule cell in treatment group was equal compared to control group. Transplantation of EPI-NCSC into hippocamp might differentiate into cholinergic neurons and could cure impairment of memory in AD rat model. Iran. Biomed. J. 16 (1): 1-9, 2012 Keyword: Alzheimer’s disease, Cholinergic neuron, Hair follicle

دریافت فایل پیوست

Iran. Biomed. J. 16 (1): 1-9, 2012

  The Role of Biodegradable Engineered Nanofiber Scaffolds Seeded with Hair Follicle Stem Cells for Tissue Engineering Iranian Biomedical Journal 16 (4): 193-201 (October 2012) ISI 5 Leila Beigom Hejazian1,2, Banafshe Esmaeilzade3, Fatima Moghanni Ghoroghi1, Fatemeh Moradi1, Marzieh Beigom Hejazian4, Anahita Aslani5, Mehrdad Bakhtiari1, Masoud Soleimani6 and Maliheh Nobakht*7,8 2 9
Background: The aim of this study was to fabricate the poly caprolactone (PCL) aligned nanofiber scaffold and to evaluate the survival, adhesion, proliferation, and differentiation of rat hair follicle stem cells (HFSC) in the graft material using electrospun PCL nanofiber scaffold for tissue engineering applications. Methods: The bulge region of rat whisker was isolated and cultured in DMEM: nutrient mixture F-12 supplemented with epidermal growth factor. The morphological and biological features of cultured bulge cells were observed by light microscopy using immunocytochemistry methods. Electrospinning was used for production of PCL nanofiber scaffolds. Scanning electron microscopy (SEM), 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, and histology analysis were used to investigate the cell morphology, viability, attachment and infiltration of the HFSC on the PCL nanofiber scaffolds. Results: The results of the MTT assay showed cell viability and cell proliferation of the HFSC on PCL nanofiber scaffolds. SEM microscopy images indicated that HFSC are attached, proliferated and spread on PCL nanofiber scaffolds. Also, immunocytochemical analysis showed cell infiltration and cell differentiation on the scaffolds. Conclusion: The results of this study reveal that PCL nanofiber scaffolds are suitable for cell culture, proliferation, differentiation and attachment. Furthermore, HFSC are attached and proliferated on PCL nanofiber scaffolds. Iran. Biomed. J. 16 (4): 193-201, 2012 Keywords: Nanofiber, Electrospinning, Stem cells, Tissue engineering

دریافت فایل پیوست

Iran. Biomed. J. 16 (4): 193-201, 2012

  Evaluation of the Effect of NT-3 and Biodegradable Poly-L-lactic Acid Nanofiber Scaffolds on Differentiation of Rat Hair Follicle Stem Cells into Neural Cells In Vitro J Mol Neurosci (2013) 51:318–327 ISI 5 Fatemeh Moghani Ghoroghi & Leila Beygom Hejazian & Banafshe Esmaielzade & Masumeh Dodel & Masoud Roudbari & Maliheh Nobakht 3 6
Recent improvement in neuroscience has led to new strategies in neural repair. Hair follicle stem cells are high promising source of accessible, active, and pluripotent adult stem cells. They have high affinity to differentiate to neurons. Aside from using cell–scaffold combinations for implantation, scaffolds can provide a suitable microenvironment for cell proliferation, migration, and differentiation. NT-3 is the most interesting neurotrophic factors being an important regulator of neural survival and differentiation. Since treatment duration in neural repair is very important, this study aims to evaluate the effect of NT- 3 and poly-L-lactic acid (PLLA) on differentiation time of bulge stem cells of rat hair follicle to neural-like cells. HFSCs of rat whisker was isolated and cultured on PLLA and differentiated with 10 ng/mL NT-3. Biological features of cultured cells were evaluated with immunocytochemistry and flowcytometry methods by using CD34, nestin, and βІІІ-tubulin markers. For cell viability and morphological assessment, MTT assay and SEM were performed. Our results showed that bulge stem cells of hair follicle can express CD34 and Nestin before differentiation. By using NT-3 during differentiation process, the cells showed positive reaction to βІІІ-tubulin antibody. MTT results demonstrated that PLLA significantly increased cell viability. Finally, HFSCs adhesion was confirmed by SEM results. The results indicate that 10 ng/mL NT-3 and PLLA have significant effect on differentiation time of rat HFSCs to neural cells even in 10 days. Keywords Bulge . Hair follicle stem cell . CD34 . Nestin . βІІІ-tubulin . NT-3 . Scaffold PLLA

دریافت فایل پیوست

J Mol Neurosci (2013) 51:318–327

  Effect of Neurotrophin-3 on differentiation of rat hair follicle stem cells into neural like cells Journal of Iranian Anatomical Sciences Vol 9, No 37, Winter 2012, page 269-278 علمی پژوهشی 3.5 Moghani Ghoroghi F., M.Sc*, Nobakht M., Ph.D., Esmaeilzade B., M.Sc., Bakhtiyari M., Ph.D., Hejazian L.B., M.Sc. 3 5
Purpose: The aim of this study was to evaluate the effect of NT-3 on the decrease of the differentiation time of bulge stem cells of rat hair follicle from neuron like cells. Materials and Methods: The bulge region of the rat whisker was isolated from and cultured in DMEM/F12 supplemented with epidermal growth factor (EGF) in 3 groups for 7,8 and 9 days . Then 10 ng /ml NT-3 was added to each group for 3 days. Finally, cell differentiation was assessed by immunocytochemistry using βⅢ-tubulin antibody and the result was compared with control groups. Results: According to our results hair follicle bulge stem cells expressed CD34 and Nestin in 7-9 and 10-12 first days after cultivation respectively. By using NT-3 duration differentiation process the cells showed positive reaction to βⅢ-tubulin antibody. Conclusion: The results indicate that NT-3 can affect on differentiation speed even in less than 10 days. Key Words: Bulge of hair follicle, CD34, Nestin, NT-3, βⅢ-tubulin

دریافت فایل پیوست

Journal of Iranian Anatomical Sciences, Vol 9, No 37, Winter 2012, page 269-278

  Neuronal Differentiation of Rat Hair Follicle Stem Cells: the Involvement of the Neuroprotective Factor Seladin-1 (DHCR24) Iranian Biomedical Journal 18 (3): 136-142 (July 2014) ISI 5 Samira Gilanchi1,2, Banafshe Esmaeilzade1,3, Akram Eidi2, Mahmood Barati4, Soraya Mehrabi5, Fatima Moghani Ghoroghi6 and Maliheh Nobakht*1,6,7 2 7
Background: The seladin-1 (selective Alzheimer disease indicator-1), also known as DHCR24, is a gene found to be down-regulated in brain region affected by Alzheimer disease (AD). Whereas, hair follicle stem cells (HFSC), which are affected in with neurogenic potential, it might to hypothesize that this multipotent cell compartment is the predominant source of seladin-1. Our aim was to evaluate seladin-1 gene expression in hair follicle stem cells. Methods: In this study, bulge area of male Wistar rat HFSC were cultured and then characterized with Seladin-1 immunocytochemistry and flow cytometry on days 8 to 14. Next, 9-11-day cells were evaluated for seladin-1 gene expression by real-time PCR. Results: Our results indicated that expression of the seladin-1 gene (DHCR24) on days 9, 10, and 11 may contribute to the development of HFSC. However, the expression of this gene on day 11 was more than day 10 and on 10th day was more than day 9. Also, we assessed HFSC on day 14 and demonstrated these cells were positive for β-ш tubulin, and seladin-1 was not expressed in this day. Conclusion: HFSC express seladin-1 and this result demonstrates that these cells might be used to cell therapy for AD in future. Iran. Biomed. J. 18 (3): 136-142, 2014 Keywords: Seladin-1 (selective Alzheimer disease indicator-1), Alzheimer disease (AD), Hair follicle stem cells

دریافت فایل پیوست

Iran. Biomed. J. 18 (3): 136-142, 2014

  Adult neurogenesis of epidermal neural crest stem cells (EPI-NCSC) in hippocampus of Alzheimer's rat model Comp Clin Pathol scopus 3.5 Banafshe Esmaeilzade & Maliheh Nobakht & Seyed Mohammad Hosseini & Pejman Mortazavi & Mahmood Barati & Soraya Mehrabi & Leila Beygom Hejazian & Fatima Moghani Ghoroghi 1 8
Abstract Alzheimer’s disease (AD) is characterised by progressive neuronal loss in the hippocampus. Our aim was to evaluate the effects of transplanting epidermal neural crest stem cells (EPI-NCSC) into the hippocampus in vivo and to assess adult neurogenesis and total granule cell number in the hippocampus of an Alzheimer’s rat model after a single injection of EPI-NCSCs. Fourteen days after a bilateral injection of β-amyloid 1–40 into the hippocampus, 10 AD model rats received an intra-hippocampal injection of EPINCSCs; the cells were obtained from the vibrissa hair follicle of the rat, cultured, labelled with bromodeoxyuridine (BrdU) and suspended in normal saline. Y-Maze and single trial passive avoidance tests were used to show any learning and memory deficit. Nestin staining was performed in vitro. Double staining of BrdU–GFAP and BrdU–βІІІ was undertaken to study survival and differentiation of the grafted cells. Cell proliferation and differentiation were observed in all part of hippocampus in the double-stained histological sections. Total granular cell number was estimated to be more per hippocampus in the rats receiving the transplanted cells compared to the AD control group. We observed that rats with hippocampal damage made significantly more errors than control rats on the Y-maze. We showed that transplanted EPI-NCSCs survived and differentiated into neurons and glial cells. Total granule cell number in the treatment group was equal to the control group. Cell proliferation and migration tends to end in the dentate gyrus and the other part of hippocampus. Transplantation of EPINCSCs into the hippocampus might differentiate into neurons or induce neurogenesis. Keyword EPI-NCSCs . Alzheimer’s disease . Granule cell number . Neurogenesis . Hippocampus

دریافت فایل پیوست

  Hair follicle stem cells: In vitro and in vivo neural differentiation World J Stem Cells 2015 June 26; 7(5): 866-872 pubmed 5 Nowruz Najafzadeh, Banafshe Esmaeilzade, Maryam Dastan Imcheh 2 3
Hair follicle stem cells (HFSCs) normally give rise to keratinocytes, sebocytes, and transient amplifying progenitor cells. Along with the capacity to proliferate rapidly, HFSCs provide the basis for establishing a putative source of stem cells for cell therapy. HFSCs are multipotent stem cells originating from the bulge area. The importance of these cells arises from two important characteristics, distinguishing them from all other adult stem cells. First, they are accessible and proliferate for long periods. Second, they are multipotent, possessing the ability to differentiate into mesodermal and ectodermal cell types. In addition to a developmental capacity in vitro , HFSCs display an ability to form differentiated cells in vivo . During the last two decades, numerous studies have led to the development of an appropriate culture condition for producing various cell lineages from HFSCs. Therefore, these stem cells are considered as a novel source for cell therapy of a broad spectrum of neurodegenerative disorders. This review presents the current status of human, rat, and mouse HFSCs from both the cellular and molecular biology and cell therapy perspectives. The first section of this review highlights the importance of HFSCs and in vitro differentiation, while the final section emphasizes the significance of cell differentiation in vivo . Key words: Hair follicle; Stem cells; Bulge area; Neuron; Differentiation

دریافت فایل پیوست

World J Stem Cells 2015 June 26; 7(5): 866-872 ISSN 1948-0210 (online)

  the cytotoxic effects of acute dose of aluminum chloride on renal filtration barrier in rabbit  The 8th Iranian anatomical sciences congress (16-19 May, 2008 Tehran/Iran Bazzy p, Esmaeilzade B, Akbarzadeh S, Zarea A 2 4
دریافت فایل پیوست

  بررسي اثر نوروتروفين 3 بركاهش زمان تمايز سلولهاي بنيادي فوليكول موي موش صحرائي به نورون  The 10th Iranian anatomical sciences congress (9-11 May, 2012 Guilan/Iran) مليحه نوبخت*، فاطمه مغني قرقي، مهرداد بختياري، بنفشه اسماعيل زاده، ليلا بيگم حجازيان 4 5
مقدمه: با توجه به پيشرفت هاي جديد در علوم اعصاب و كشت سلولي، درمانهاي جديد براي ترميم عصبي پيش رو قرار گرفته است. در اين راستا استفاده از سلولهاي بنيادي براي تمايز به نورونها مورد توجه قرار گرفته اند. براي اين منظور استفاده فوليكول موبه دليل دسترسي آسان و چند ظرفيتي بودن و نداشتن موارد bulge از سلولهاي بنيادي بالغ موجود در ناحيه نيز به عنوان يكي NT- اخلاقي سلولهاي بنيادي جنيني و پتانسيل تمايز به سلولهاي عصبي از اهميت ويژه اي برخوردارند. 3 از فاكتورهاي تروفيك شناخته شده سيستم عصبي به جهت ايفاي نقش مهم تسهيل در زنده ماندن و تمايز نوروني در طي تكامل بسيار مورد توجه مي باشد. از آنجائيكه كوتاه كردن زمان ترميم عصبي فاكتور بسيار مهمي در روند درمان مي باشد، فوليكول مو به نورون مي باشد. bulge بر كاهش زمان تمايز سلولهاي بنيادي ناحيه NT- هدف از اين پژوهش بررسي تأثير 3 آن كشت داده شد. سلولهاي bulge مواد و روش ها: ابتداء فوليكولهاي ناحيه پشت لب موش صحرائي جداشده و ناحيه در 3 گروه كشت داده شدند. در اين 3 گروه ، EGF حاوي DMEM/F در محيط 12 bulge بنيادي بدست آمده از ناحيه با دوز 10 نانوگرم در ميلي ليتر قرار NT- پاساژ در روزهاي 7،8 و 9 انجام شد .سپس هر گروه به مدت 3 روز در مجاورت 3 در اين سه گروه (βⅢtubulin) داده شد. سپس بوسيله تكنيك ايمونو سيتوشيمي تمايز سلولها با آنتي بادي بتاتري توبولين مورد بررسي قرار گرفت و با گروه كنترل مقايسه شد. 10- و در طي 12 CD 7 روز اول ماركر 34 - فوليكول در 9 bulge نتايج: در اين مطالعه سلولهاي بنيادي بدست آمده از ناحيه به مدت 3 روز در گروههاي ذكر شده همزمان با روند تمايز، NT- روز اول ماركر نستين را بيان كردند. سپس پس از دريافت 3 واكنش مثبت نشان دادند. ( βⅢtubulin) اين سلولها با آنتي بادي بتاتري توبولين بر تسريع روند تمايز سلولهاي بنيادي فوليكول مو اثر معني داري دارد و NT- نتيجه گيري: با توجه به نتايج اين مطالعه، 3 را به نورون متمايز كند. bulge حتي مي تواند در كمتر از 10 روز سلولهاي بنيادي ناحيه بتاتري توبولين ،NT- نستين ، 3 ،CD فوليكول مو، 34 ، bulge : واژه هاي كليدي

دریافت فایل پیوست

  پيوند سلولهاي بنيادي فوليكول مو سبب نوروژنز در هيپوكمپ موشهاي صحرايي نر مدل آلزايمر مي شود  The 10th Iranian anatomical sciences congress (9-11 May, 2012 Guilan/Iran) بنفشه اسمعيل زاده*، مليحه نوبخت، سيد محمد حسيني، ليلا بيگم حجازيان، فاطمه مغني قرقي 1 5
هدف از اين تحقيق ارزيابي اثر پيوند سلولهاي بنيادي برنوروژنز در هيپو كمپ موشهاي صحرايي مدل آلزايمر بود. با تزريق 1 درهيپوكمپ دو طرف موشهاي صحرايي مدل آلزايمر ايجاد شد، 2 هفته بعد از تزريق براي نشان دادن - بتا– آميلوئيد 40 اختلال در حافظه و ياد گيري تست رفتاري انجام شد. سلولهاي بنيادي فوليكول مو از فوليكول موي سبيل موش صحرايي جدا ليبل شد. بعد از اثبات ايجاد آلزايمر سلولهاي بنيادي فوليكول موبه ناحيه هيپوكمپ پيوند زده شد. مار BrdU كشت داده و با كرهاي اختصاصي اين سلولها با فلوسايتومتري اندازه گيري شد. با رنگ آميزي ايمونوفلورسانس دو گانه بقا سلولهاي پيوند زده شده وتمايز آنها به نورون وگلياها نشان داده شد.شمارش سلولهاي گرانولر هيپوكمپ در گروه گيرنده پيوند افزايش معني داري نسبت به گروه آلزايمر كنترل داشت كه نتيجه گرفته شد علاوه بر تمايز، ناشي از تحريك نوروژنز در هيپوكمپ است .همچنين مشاهده شد گروه گيرنده پيوند به طور معني داري در تست رفتاري مجدد اشتباهات كمتري نسبت به گروه آلزايمر كنترل داشت. واژه هاي كليدي: سلولهاي بنيادي فوليكول مو، بيماري آلزايمر، تست رفتاري ، نوروژنز

دریافت فایل پیوست

  پيوند سلولهاي بنيادي فوليكول مو سبب نوروژنز در هيپوكمپ موشهاي صحرايي نر مدل آلزايمر مي شود  The 10th Iranian anatomical sciences congress (9-11 May, 2012 Guilan/Iran) oral presentation : بنفشه اسمعيل زاده*، مليحه نوبخت، سيد محمد حسيني، ليلا بيگم حجازيان، فاطمه مغني قرقي 1 5
هدف از اين تحقيق ارزيابي اثر پيوند سلولهاي بنيادي برنوروژنز در هيپو كمپ موشهاي صحرايي مدل آلزايمر بود. با تزريق 1 درهيپوكمپ دو طرف موشهاي صحرايي مدل آلزايمر ايجاد شد، 2 هفته بعد از تزريق براي نشان دادن - بتا– آميلوئيد 40 اختلال در حافظه و ياد گيري تست رفتاري انجام شد. سلولهاي بنيادي فوليكول مو از فوليكول موي سبيل موش صحرايي جدا ليبل شد. بعد از اثبات ايجاد آلزايمر سلولهاي بنيادي فوليكول موبه ناحيه هيپوكمپ پيوند زده شد. مار BrdU كشت داده و با كرهاي اختصاصي اين سلولها با فلوسايتومتري اندازه گيري شد. با رنگ آميزي ايمونوفلورسانس دو گانه بقا سلولهاي پيوند زده شده وتمايز آنها به نورون وگلياها نشان داده شد.شمارش سلولهاي گرانولر هيپوكمپ در گروه گيرنده پيوند افزايش معني داري نسبت به گروه آلزايمر كنترل داشت كه نتيجه گرفته شد علاوه بر تمايز، ناشي از تحريك نوروژنز در هيپوكمپ است .همچنين مشاهده شد گروه گيرنده پيوند به طور معني داري در تست رفتاري مجدد اشتباهات كمتري نسبت به گروه آلزايمر كنترل داشت. واژه هاي كليدي: سلولهاي بنيادي فوليكول مو، بيماري آلزايمر، تست رفتاري ، نوروژنز

دریافت فایل پیوست


< >